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| Registros recuperados: 425 | |
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| Dundon, William G.; Arzul, Isabelle; Omnes, Emmanuelle; Robert, Maeva; Magnabosco, Cristian; Zambon, Michela; Gennari, Lorenzo; Toffan, Anna; Terregino, Calogero; Capua, Ilaria; Arcangeli, Giuseppe. |
| In April 2010, the sampling of juvenile Pacific cupped oysters originating from France was undertaken from a farm located off the coast of the Marche region in Italy. Samples were sent to the national reference laboratory for mollusc diseases for bacteriological, histological, electron microscopical and molecular analysis. Classical and Real-Time PCR indicated the presence of the microvariant (OsHV-1 mu var) of Type 1 Ostreid Herpes virus (OsHV-1) despite the absence of clinical and pathological signs normally associated with the presence of this variant. Further molecular and sequencing analysis revealed the presence of OsHV-1 par and OsHV-1 DNA in one oyster indicating that simultaneous detection of both viruses is possible. This is the first report of... |
| Tipo: Text |
Palavras-chave: Pacific cupped oyster; Ostreid Herpes virus 1 mu var; PCR; Italy. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00035/14585/11968.pdf |
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| Oliveira,Sílvia Dias de; Rodenbusch,Carla Rosane; Michael,Geovana B.; Cardoso,Marisa I.R.; Canal,Cláudio Wageck; Brandelli,Adriano. |
| The presence of three virulence genes, invA, spvR, and spvC, was determined in Salmonella Enteritidis isolated from poultry, pigs, humans and food. All isolates were positive for the invA gene, with 91.2% being positive for spvR and 90.2% for spvC. There was no significant difference in the prevalence of the virulence genes between isolates from different sources. The results indicate that there is a putative high virulence potential for the S. Enteritidis isolates characterized. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella Enteritidis; Virulence; Spv; PCR. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500042 |
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| Rueda Zozaya, Rocío del Pilar. |
| En México se encuentran los felinos más grandes de América, el puma (Puma concolor) y el jaguar (Panthera onca). Debido al incremento en las fronteras agropecuarias, el jaguar y el puma se encuentran en peligro de extinción en varias áreas de su distribución. Aunada a su situación crítica, su comportamiento elusivo y críptico dificulta aún más la accesibilidad para realizar investigación acerca de su etología, hábitos alimenticios y estimación de parámetros poblacionales. Por ello, se ha tenido la necesidad de recurrir a otros métodos de investigación no invasivos que eviten el manejo directo, uno de estos métodos es la recolección de excretas para identificar los componentes de su dieta, con esta información, se puede evaluar si realmente estos felinos... |
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Palavras-chave: Panthera onca; Puma concolor; Dieta; PCR; Diet; Ganadería; Fauna silvestre; Maestría. |
| Ano: 2010 |
URL: http://hdl.handle.net/10521/76 |
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| Rueda Zozaya, Rocío del Pilar. |
| En México se encuentran los felinos más grandes de América, el puma (Puma concolor) y el jaguar (Panthera onca). Debido al incremento en las fronteras agropecuarias, el jaguar y el puma se encuentran en peligro de extinción en varias áreas de su distribución. Aunada a su situación crítica, su comportamiento elusivo y críptico dificulta aún más la accesibilidad para realizar investigación acerca de su etología, hábitos alimenticios y estimación de parámetros poblacionales. Por ello, se ha tenido la necesidad de recurrir a otros métodos de investigación no invasivos que eviten el manejo directo, uno de estos métodos es la recolección de excretas para identificar los componentes de su dieta, con esta información, se puede evaluar si realmente estos felinos... |
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Palavras-chave: Panthera onca; Puma concolor; Dieta; PCR; Diet; Ganadería; Fauna silvestre; Maestría. |
| Ano: 2010 |
URL: http://hdl.handle.net/10521/76 |
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| Xuan, Xuenan; Igarashi, Ikuo; Avarzed, A.; Ikadai, Hiromi; Inoue, Noboru; Nagasawa, Hideyuki; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男; 井上, 昇. |
| A set of primers were designed according to the published sequence of the gene encoding a rhoptry protein of Babesia caballi, and used to amplify parasite DNA from the blood samples obtained from carrier horses by polymerase chain reaction（PCR）method．The PCR method was sensitive enough to detect parasite DNA from 2.5 μl blood sample with a parasitemia of 0.000001％. The PCR method was compared with fluorescent antibody test(IFAT) in order to evaluate the diagnosis effciency for B. caball infection in horses. Of 142 field samples from Mongolia, 28(20%) and 96(69%)samples were identified positively by PCR and IFAT, respectively. Although the sensitivity of PCR was lower than IFAT, it was noted that the 5 IFAT-negative samples were PCR-positive, suggesting... |
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Palavras-chave: Babesia caballi; PCR; IFAT. |
| Ano: 1998 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/281 |
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| Costa,E.A.; Sant'Ana,F.M; Carvalho,C.J.S.; Moustacas,V.S.; Silva,S.M.M.S.; Paixão,T.A.; Santos,R.L.. |
| A brucelose ovina causada pela Brucella ovis é uma doença reprodutiva de carneiros caracterizada por epididimite, orquite, com consequente diminuição da fertilidade e prejuízos econômicos significativos. O presente trabalho teve por objetivo avaliar a aplicabilidade da técnica de PCR como um método de diagnóstico em campo, comparado-a com a técnica de IDGA. Foram coletadas amostras de urina e soro de 90 carneiros oriundos de 31 rebanhos localizados no Estado do Piauí. Quatro das 31 (12,9%) propriedades avaliadas apresentaram animais positivos. Dezoito (20%) amostras de urina foram positivas pela PCR, enquanto o método de IDGA identificou 16 (17,8%) carneiros soropositivos. Embora os métodos tenham apresentado concordância baixa na estatística Kappa... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucella ovis; Sheep; Diagnosis; PCR; AGID. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000300029 |
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| Yaman,S; Sahan Yapicier,O. |
| ABSTRACT Gallibacterium anatis, a member of the Pasteurellaceae family, leads to decrease in egg-production, animal welfare and increase in mortality. This study aimed to diagnose G. Anatis, which caused economic losses in laying hens by using conventional and molecular techniques. In this study, G. anatis was examined from a total of 200 dead chicken tissues (heart, liver, lung, spleen and trachea) in laying hen farms that observed a decrease in egg production with respiratory system infection. Conventional methods based on colony morphology, sugar fermentation tests and hemolytic properties and molecular conformation using 16S rRNA-23S rRNA specific primers were performed to identify G. anatis. G. anatis was isolated in 20 (10%) of the examined samples... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gallibacterium anatis; Isolation and identification; Layers; PCR. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300307 |
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| Cezarino,Bruno Nicolino; Yamamoto,Lidia; Del Negro,Gilda Maria Barbaro; Rocha,Daisy; Okay,Thelma Suely. |
| Group B streptococcus (GBS) remains the most common cause of early-onset sepsis in newborns. Laboratory gold-standard, broth culture methods are highly specific, but lack sensitivity. The aim of this study was to validate a nested-PCR and to determine whether residue volumes of urine samples obtained by non invasive, non sterile methods could be used to confirm neonatal GBS sepsis. The nested-PCR was performed with primers of the major GBS surface antigen. Unavailability of biological samples to perform life supporting exams, as well as others to elucidate the etiology of infections is a frequent problem concerning newborn patients. Nevertheless, we decided to include cases according to strict criteria: newborns had to present with signs and symptoms... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GBS; Neonatal sepsis; Early-onset neonatal sepsis; Neonatal infection; PCR; Nested-PCR. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100005 |
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| Silva,Rosemeri Maurici da; Machado,Tairone; Bazzo,Maria Luiza. |
| This study was performed to assess the efficiency of polymerase chain reaction (PCR) directly from sputum for the diagnosis of pulmonary tuberculosis by comparison between HIV-positive and HIV-negative individuals. Sputum samples were collected from hospitalized patients admitted with a clinical diagnosis of pulmonary tuberculosis, and subjected to smear microscopy, culture on LJ medium and detection of M. tuberculosis by PCR. Sensitivity, specificity, and predictive values (positive and negative) were calculated using smear and/or culture at day 42 as the gold standard, by comparing the yield in HIV-positive and HIV-negative individuals. Regardless of serostatus, the technique's yield had 62% sensitivity, 70% specificity, 79% positive predictive value,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; HIV; PCR; Sputum. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100030 |
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| Carvalho, Fábio Santos; Rocha, Josiane Moreira; Wenceslau, Amauri Arias; Oliveira, Haniel Cedraz de; Santos, Ivanildo dos Anjos; Gross, Eduardo. |
| A Bahia é o estado da região Nordeste com maior número de registros de leishmaniose em humanos.Os cães também podem se infectar e tem importante papel na manutenção da doença no ambiente urbano. Sendo assim, objetivou-se investigar a ocorrência de leishmaniose visceral canina em animais das unidades de Centro de Controle de Zoonoses (CCZ) da Bahia utilizando técnicas sorológicas e moleculares. Foram examinados 100 cães dos CCZ dos municípios Barreiras, Eunápolis, Ilhéus, Itabuna e Feira de Santana. Após exame clínico dos animais coletou-se 10 mL de sangue da veia jugular para avaliação hematológica, extração de DNA e obtenção de soro. O DNA genômico foi extraído utilizando-se fenol:clorofórmio:álcool isoamilico (25:24:1). O diagnóstico sorológico foi... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: L. infantum; ELISA; PCR; Cães. |
| Ano: 2015 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/25304 |
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| Iglesia,Aleika; Alvarez,Elba; Martínez,Yamila; García,A. |
| La marchitez bacteriana causada por Ralstonia solanacearum, es una de las enfermedades más importante que afecta a una amplia gama de cultivos en las regiones tropicales y subtropicales. En Cuba esta enfermedad se encuentra bajo estrictas medidas de cuarentena, sin embargo el riesgo de entrada crece cada año debido a la importación de material vegetal procedente de zonas de altos niveles de infestación. En este trabajo se introduce y desarrolla el uso de la reacción en cadena de la polimerasa (PCR) para la detección y confirmación de R. solanacearum en materiales importados, así como en la vigilancia de la enfermedad en el sistema nacional de sanidad vegetal. La metodología utilizada facilitó la detección de R. solanacearum en cultivos de células hasta... |
| Tipo: Journal article |
Palavras-chave: Cuarentena; Diagnóstico molecular; Ralstonia solanacearum; PCR. |
| Ano: 2008 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522008000200002 |
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| Rocha, Josiane Moreira; Carvalho, Fabio Santos; Oliveira, Haniel Cedraz de; Munhoz, Alexandre Dias; Wenceslau, Amauri Arias. |
| Objetivou-se diagnosticar Erlichia canis com o emprego de dois métodos: i) molecular (PCR) e; ii) parasitológico (imprint em lâmina). Para tanto, foram selecionados cães com alterações clínicas, hematológicas e bioquímicas (febre, petéquias, alterações de linfonodos periféricos, apatia, trombocitopenia, anemia e pancitopenia e presença de carrapatos). Foram coletadas amostras de 10ml de sangue de 287 cães de seis bairros do município de Ilhéus, Bahia. Foi realizado o esfregaço de sangue de ponta de orelha em lâminas coradas por kit Panótico rápido, hemograma completo, realizado em contador automático ABCVet (Animal Blood Counter) utilizando kit ABX Vetpack (HURIBA). Parte do sangue foi centrifugado para obtenção do soro e verificação dos níveis de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Erliquiose; PCR; Cão. |
| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/21342 |
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| Quiles,Milene Gonçalves; Boettger,Bruno Cruz; Inoue,Fernanda Matsiko; Monteiro,Jussimara; Santos,Daniel Wagner; Ponzio,Vinicius; Carlesse,Fabianne; Cappellano,Paola; Carvalhaes,Cecilia Godoy; Pignatari,Antonio Carlos Campos. |
| ABSTRACT Bloodstream infections (BSIs) are serious infections associated with high rates of morbidity and mortality. Every hour delay in initiation of an effective antibiotic increases mortality due to sepsis by 7%. Turnaround time (TAT) for conventional blood cultures takes 48 h, forcing physicians to streamline therapy by exposing patients to broad-spectrum antimicrobials. Our objective was (1) to evaluate the accuracy and TAT of an optimized workflow combining direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and in-house real-time polymerase chain reaction (PCR) for bacterial identification and antimicrobial resistance profiling directly from positive blood bottles for diagnosing bloodstream infections... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bloodstream infections; Molecular diagnosis; Molecular panels; PCR; Mass spectrometry. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000300164 |
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| Sircili,Marcelo Palma; Roxo,Eliana; Leão,Sylvia Cardoso. |
| Mycobacterium avium complex (MAC) species cannot be discriminated by the usual methods of biochemical identification of mycobacteria. This study showed that amplification by PCR of DT1 and DT6, two single copy sequences identified in the genome of M. avium serotype 2, the insertion sequence IS1245, found to be consistently present in M. avium strains and the heat-shock protein gene hsp65, followed by restriction polymorphism analysis, are rapid and accurate tests for the differentiation of the species M. avium, M. intracellulare, and M. scrofulaceum. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Amplification; PCR; MAC; Mycobacterium; Identification. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200011 |
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| Pinhal,Danillo; Gadig,Otto BF; Wasko,Adriane P; Oliveira,Claudio; Ron,Ernesto; Foresti,Fausto; Martins,Cesar. |
| Sharks are suffering from intensive exploitation by worldwide fisheries leading to a severe decline in several populations in the last decades. The lack of biological data on a species-specific basis, associated with a k-strategist life history make it difficult to correctly manage and conserve these animals. The aim of the present study was to develop a DNA-based procedure to discriminate shark species by means of a rapid, low cost and easily applicable PCR analysis based on 5S rDNA repeat units amplification, in order to contribute conservation management of these animals. The generated agarose electrophoresis band patterns allowed to unequivocally distinguish eight shark species. The data showed for the first time that a simple PCR is able to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chondrichthyes; PCR; Species identification; 5S rDNA; Sharks. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200033 |
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| Registros recuperados: 425 | |
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